ABSTRACT
El objetivo de este trabajo es aumentar la sensibilidad de la detección de antígeno de VIH en pacientes infectados a través de un proceso de disociación de complejos inmunes que no reduzca la reactividad antigénica. Se realizó una etapa de precipitación con PEG 12 por ciento (concentración final 2 por ciento), tratamiento ácido disociante y neutralización, previo a la aplicación de un método convencional de captura de antígeno. Se mostró una relación significativa entre el tratamiento con PEG disociante y la detección de antígeno (X2: 13,97, p < 0,001). De 105 muestras, 27 sueros que resultaron negativos por el método estándar fueron positivos con el tratamiento. La cantidad de antígeno en 35 muestras previamente positivas se incrementó en promedio 2,3 veces luego del tratamiento
Subject(s)
Humans , HIV Antigens/isolation & purification , Antigen-Antibody Complex/immunology , Polyethylene Glycols , Sensitivity and Specificity , Acquired Immunodeficiency Syndrome/diagnosis , ArgentinaABSTRACT
An acute clinical picture of variable intensity may occur during the initial primary phase of HIV infection, it may however pass unnoticed. We report 12 seronegative subjects (11 male homosexuals, 1 female heterosexual, aged 18 to 44 years old), that sembling an acute clinical picture preceding seroconversion. All had a sudden beginning, reduration were variable, lasting a mean of 14 (range 5-44) days an remaining asymptomatic thereafter. Most patients presented a discrete leukopenia with lymphopenia at the expense of CD4 lymphocytes, followed by an absolute lymphocytosis in some, with an increase in CD8 lymphocytes. All became positive for HIV; circulating HIV antigen was identified in 3 and IgM anti-HIV antibodies were detected during the symptomatic period by third generation ELISA in other 3. It is concluded that the clinical picture of primary HIV infection has identificable clinical serological and immunological features and its recognition has diagnostic and preventive implications